In SubCh1_TrainingData, for each isolate why is the DHA_IC50 value the same for every timepoint, treatment and replicate ?
Sorry that this is likely a stupid question!
Created by mark_l Many thanks for your detailed response, Sage Hi mark_l,
Thanks for the question.
The DHA_IC50 value is the same for every isolate because it is measured as a separate experiment from the transcriptional data. As such, the DHA_IC50 is unique to each isolate, not every timepoint, treatment and replicate.
Briefly, a culture is is exposed to a gradient to varying DHA concentrations for 72 hours starting at early ring stage (0~12 hours post invasion) and the concentration at which 50% of the parasites growth is inhibited is determined. This is done for every isolate in triplicate and the average is reported in the DHA_IC50 column.
For more information about the IC50 protocol, I recommend [https://www3.nd.edu/~bsmith3/pdf/BMC2017.pdf], specifically section 4.4. Hypoxanthine incorporation growth inhibition assay.
I hope this answers your question.
If you have any follow up questions, I'll be more than happy to answer them.
Thanks.
-Sage
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