url: https://www.jax.org/strain/030922 data: Jax.IU.Pitt_PrimaryScreen Jax.IU.Pitt_StrainValidation grant: U54AG054345 program: MODEL-AD species: Mouse summary: CRISPR/Cas9 was used to engineer a point mutation in the Mthfr gene (GCC->GTC) that models the human C677T polymorphism and causes the A262V amino acid substitution. This models the late-onset AD risk variant SNP rs1801133. This Mthfr variant is expressed on a sensitized model also expressing a humanized APOE4 knock-in and a CRISPR/cas9-generated R47H point mutation in the mouse Trem2 gene. AlzForum: [Link](https://www.alzforum.org/research-models/mthfrc677tapoe4trem2r47h) toolType: Mouse Model modelType: Late Onset Alzheimer's disease contributor: Mike Sasner availableData: MthfrC677TSNP strainDetails: [View](https://www.jax.org/strain/030922) targetedGenes: MTHFR APOE TREM2 backgroundStrain: C57BL/6J alzforumInformation: [View](https://www.alzforum.org/research-models/mthfrc677tapoe4trem2r47h)