Hello, I have downloaded the .bam files from https://doi.org/10.7303/syn4164376 and wish to quantify them using SALMON; however, they do not seem to map to the GENCODE hg19 release 14 reference transcript fasta file as written in https://adknowledgeportal.synapse.org/Explore/Studies/DetailsPage/StudyDetails?Study=syn3219045. May I ask how these .bam files were generated with what mapping reference/tools used? Thanks!

Created by Young-Jun Jeon jeonrpm2020
@jeonrpm2020 What error are you getting when aligning the reads? (low alignment rate, software) More information may help

What transcriptome reference are the RNA-seq (bulk brain) .bam files mapped to? page is loading…