Hi, Is the numbering system used for these samples is the same as that of the hg19? I get the sequences according to the bed file through bedtools, but found many unmatched nucleotides. For example, for the mutation "chr18:42456670C>CTCTT" of sample UniPD_2348_01. I found that mutation should be on the gene SETBP1 (AMPL7154375056, chr18: 42456461~42456784), but based on the sequence from hg19 the reference at position 42456670 should be A rather than C. I tried to add or minus 1 on the positions as sometimes the starting index is 0 rather than 1, but then there would be other errors. Therefore, could you please help solve this problem? Thank you!

Created by Shaowen Zhu ShaowenZ

Numbering system of the samples. page is loading…