DOI: 10.1007/978-1-4939-7768-0_12 year: 2019 study: ASD-lncRNA title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq). grants: R21MH105881 authors: Rhie SK, Schreiner S, Farnham PJ journal: Methods in molecular biology (Clifton N.J.) abstract: NOMe-seq (nucleosome occupancy and methylome sequencing) identifies nucleosome-depleted regions that correspond to promoters, enhancers, and insulators. The NOMe-seq method is based on the treatment of chromatin with the M.CviPI methyltransferase, which methylates GpC dinucleotides that are not protected by nucleosomes or other proteins that are tightly bound to the chromatin (GpC<sup>m</sup> does not occur in the human genome and therefore there is no endogenous background of GpC<sup>m</sup>). pubmedId: 29605855 entity_name: Rhie Methods in molecular biology (Clifton N.J.) 2019 (Pubmed ID 29605855)
Drop files to upload
Rhie Methods in molecular biology (Clifton N.J.) 2019 (Pubmed ID 29605855) page is loading…