Id: e6af6af3-4996-4d44-a56e-7cf884244095 eTag: 97e003c2-6465-4807-85b1-8816ff234cbe EntityId: syn52383428 entityId: syn52383428 Component: DatasetView DatasetUrl: https://www.ebi.ac.uk/pride/archive/projects/PXD038327 DatasetName: CSE1L and NUP37 proximity labeling in MCF10A cells expressing chimeric ErbB1:ErbB2 receptors primary_key: DatasetView_id DatasetAlias: PXD038327 DatasetAssay: Proteomics Assay, Proximity Ligation Assay DatasetDesign: Cells were cultured with reconstituted basement membrane, BirA* alleles induced with doxycycline, ErbB receptors dimerized with a synthetic small molecule, and biotinylation was performed for 24 hours. Lysates underwent a two-step biotin affinity purification before gel electrophoresis and mass spectrometry on selected gel fragments. DatasetTissue: Breast, Epithelium DatasetViewId: PXD038327 DatasetSpecies: Cat DatasetPubmedId: 37055441 DatasetTumorType: Breast Neoplasm DatasetDescription: This project uses an MCF10A clone (B2B1) expressing chimeric synthetic receptors for ErbB1 (EGFR) and ErbB2 (HER2) along with BirA*-fused inducible alleles of CSE1L or NUP37. DatasetFileFormats: RAW, mzIdentML, MGF DatasetGrantNumber: CA274499
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